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lambda uni-zap xr vector–bluescript sk phagemid system  (Agilent technologies)


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    Agilent technologies lambda uni-zap xr vector–bluescript sk phagemid system
    Lambda Uni Zap Xr Vector–Bluescript Sk Phagemid System, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lambda+uni-zap+xr+vector/pmc03073943-176-17-33
    Average 90 stars, based on 1 article reviews
    lambda uni-zap xr vector–bluescript sk phagemid system - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Isolation:

    Article Title: Defining the phosphite-regulated transcriptome of the plant pathogen Phytophthora cinnamomi.
    Article Snippet: Phosphite, an analog of phosphate is used to control oomycete diseases on a wide range of horticultural crops and in native ecosystems.. In this study, we investigated morphological and transcriptional changes induced in Phytophthora cinnamomi by phosphite.. Cytological observations revealed that phosphite caused hyphal distortions and lysis of cell walls and had an adverse effect on hyphal growth.

    Article Title: Characterization of a novel Taenia solium oncosphere antigen
    Article Snippet: The cDNA was cloned into the lambda Uni-ZAP XR vector followed by packaging of the lambda DNA using the Gigapack® III Gold Cloning Kit (Stratagene) according to manufacturer’s instructions.

    Article Title: Hamster sperm protein, p26h: a member of the short-chain dehydrogenase/reductase superfamily.
    Article Snippet: In brief, testicular poly(A)1 RNA was reverse transcribed and ligated into the lambda Uni-Zap XR vector (Stratagene, La Jolla, CA).

    Article Title: A genomic survey of the fish parasite Spironucleus salmonicida indicates genomic plasticity among diplomonads and significant lateral gene transfer in eukaryote genome evolution
    Article Snippet: Messenger RNA from approximately 10 8 cells of S. salmonicida was isolated using the Dynabead mRNA system (Dynal), and cDNA was synthesized and cloned into the lambda Uni-Zap XR vector (Stratagene), according to the manufacturer's instructions.

    Article Title: Characterisation of tumour-associated antigens in colon cancer.
    Article Snippet: In order to search for clinically relevant cancerassociated genes and to define further the spectrum of immunogenic proteins, we applied SEREX (serological identification of antigens by recombinant expression cloning) to analyse genes expressed in colon adenocarcinoma.. Eight different serum-reactive cDNA clones were isolated by immunoscreening froma colon cancer-derived cDNA expression library. mRNA expression studies showed that 2 of them, RHAMM and AD034, have a differential tissue distribution, and that 3 genes, NAP1L1, RHAMM and AD034, are overexpressed in tumours in comparison with the adjacent non-cancerous tissues.. 5¢ RLM-RACE analysis of AD034, a sequence with a tyrosine kinase motif, revealed a frameshifting insertion of 32 bp, most likely generated by use of cryptic splice site in tumour-derived cDNA.

    Article Title: The barley serine/threonine kinase gene Rpg1 providing resistance to stem rust belongs to a gene family with five other members encoding kinase domains.
    Article Snippet: The barley (Hordeum vulgare L.) stem rust (Puccinia graminis f. sp. tritici) resistance gene Rpg1 encodes a serine/threonine protein kinase with two tandem kinase domains.. The Rpg1 gene family was identiWed from the cv.. Morex and consists of Wve additional members with divergent homology to Rpg1.

    Article Title: Expression of a Novel Leishmania Gene Encoding a Histone H1-Like Protein in Leishmania major Modulates Parasite Infectivity In Vitro
    Article Snippet: An L. major cDNA library was constructed in the lambda Uni-ZAP XR vector as described in the technical manual provided by Stratagene Inc. (La Jolla, Calif.).

    Article Title: Genes expressed in zoospores of Phytophthora nicotianae.
    Article Snippet: The genus Phytophthora includes many highly destructive plant pathogens.. In many Phytophthora species, pathogen dispersal and initiation of plant infection are achieved by motile, biflagellate zoospores that are chemotactically attracted to suitable infection sites.. In order to study gene expression in zoospores, we have constructed a cDNA library using mRNA from zoospores of Phytophthora nicotianae.

    Synthesized:

    Article Title: Defining the phosphite-regulated transcriptome of the plant pathogen Phytophthora cinnamomi.
    Article Snippet: Phosphite, an analog of phosphate is used to control oomycete diseases on a wide range of horticultural crops and in native ecosystems.. In this study, we investigated morphological and transcriptional changes induced in Phytophthora cinnamomi by phosphite.. Cytological observations revealed that phosphite caused hyphal distortions and lysis of cell walls and had an adverse effect on hyphal growth.

    Article Title: Characterization of a novel Taenia solium oncosphere antigen
    Article Snippet: The cDNA was cloned into the lambda Uni-ZAP XR vector followed by packaging of the lambda DNA using the Gigapack® III Gold Cloning Kit (Stratagene) according to manufacturer’s instructions.

    Article Title: Hamster sperm protein, p26h: a member of the short-chain dehydrogenase/reductase superfamily.
    Article Snippet: In brief, testicular poly(A)1 RNA was reverse transcribed and ligated into the lambda Uni-Zap XR vector (Stratagene, La Jolla, CA).

    Article Title: A genomic survey of the fish parasite Spironucleus salmonicida indicates genomic plasticity among diplomonads and significant lateral gene transfer in eukaryote genome evolution
    Article Snippet: Messenger RNA from approximately 10 8 cells of S. salmonicida was isolated using the Dynabead mRNA system (Dynal), and cDNA was synthesized and cloned into the lambda Uni-Zap XR vector (Stratagene), according to the manufacturer's instructions.

    Article Title: Characterisation of tumour-associated antigens in colon cancer.
    Article Snippet: In order to search for clinically relevant cancerassociated genes and to define further the spectrum of immunogenic proteins, we applied SEREX (serological identification of antigens by recombinant expression cloning) to analyse genes expressed in colon adenocarcinoma.. Eight different serum-reactive cDNA clones were isolated by immunoscreening froma colon cancer-derived cDNA expression library. mRNA expression studies showed that 2 of them, RHAMM and AD034, have a differential tissue distribution, and that 3 genes, NAP1L1, RHAMM and AD034, are overexpressed in tumours in comparison with the adjacent non-cancerous tissues.. 5¢ RLM-RACE analysis of AD034, a sequence with a tyrosine kinase motif, revealed a frameshifting insertion of 32 bp, most likely generated by use of cryptic splice site in tumour-derived cDNA.

    Article Title: The barley serine/threonine kinase gene Rpg1 providing resistance to stem rust belongs to a gene family with five other members encoding kinase domains.
    Article Snippet: The barley (Hordeum vulgare L.) stem rust (Puccinia graminis f. sp. tritici) resistance gene Rpg1 encodes a serine/threonine protein kinase with two tandem kinase domains.. The Rpg1 gene family was identiWed from the cv.. Morex and consists of Wve additional members with divergent homology to Rpg1.

    Article Title: Expression of a Novel Leishmania Gene Encoding a Histone H1-Like Protein in Leishmania major Modulates Parasite Infectivity In Vitro
    Article Snippet: An L. major cDNA library was constructed in the lambda Uni-ZAP XR vector as described in the technical manual provided by Stratagene Inc. (La Jolla, Calif.).

    Article Title: Genes expressed in zoospores of Phytophthora nicotianae.
    Article Snippet: The genus Phytophthora includes many highly destructive plant pathogens.. In many Phytophthora species, pathogen dispersal and initiation of plant infection are achieved by motile, biflagellate zoospores that are chemotactically attracted to suitable infection sites.. In order to study gene expression in zoospores, we have constructed a cDNA library using mRNA from zoospores of Phytophthora nicotianae.

    Clone Assay:

    Article Title: Defining the phosphite-regulated transcriptome of the plant pathogen Phytophthora cinnamomi.
    Article Snippet: Phosphite, an analog of phosphate is used to control oomycete diseases on a wide range of horticultural crops and in native ecosystems.. In this study, we investigated morphological and transcriptional changes induced in Phytophthora cinnamomi by phosphite.. Cytological observations revealed that phosphite caused hyphal distortions and lysis of cell walls and had an adverse effect on hyphal growth.

    Article Title: Characterization of a novel Taenia solium oncosphere antigen
    Article Snippet: The cDNA was cloned into the lambda Uni-ZAP XR vector followed by packaging of the lambda DNA using the Gigapack® III Gold Cloning Kit (Stratagene) according to manufacturer’s instructions.

    Article Title: Hamster sperm protein, p26h: a member of the short-chain dehydrogenase/reductase superfamily.
    Article Snippet: In brief, testicular poly(A)1 RNA was reverse transcribed and ligated into the lambda Uni-Zap XR vector (Stratagene, La Jolla, CA).

    Article Title: A genomic survey of the fish parasite Spironucleus salmonicida indicates genomic plasticity among diplomonads and significant lateral gene transfer in eukaryote genome evolution
    Article Snippet: Messenger RNA from approximately 10 8 cells of S. salmonicida was isolated using the Dynabead mRNA system (Dynal), and cDNA was synthesized and cloned into the lambda Uni-Zap XR vector (Stratagene), according to the manufacturer's instructions.

    Article Title: Characterisation of tumour-associated antigens in colon cancer.
    Article Snippet: In order to search for clinically relevant cancerassociated genes and to define further the spectrum of immunogenic proteins, we applied SEREX (serological identification of antigens by recombinant expression cloning) to analyse genes expressed in colon adenocarcinoma.. Eight different serum-reactive cDNA clones were isolated by immunoscreening froma colon cancer-derived cDNA expression library. mRNA expression studies showed that 2 of them, RHAMM and AD034, have a differential tissue distribution, and that 3 genes, NAP1L1, RHAMM and AD034, are overexpressed in tumours in comparison with the adjacent non-cancerous tissues.. 5¢ RLM-RACE analysis of AD034, a sequence with a tyrosine kinase motif, revealed a frameshifting insertion of 32 bp, most likely generated by use of cryptic splice site in tumour-derived cDNA.

    Article Title: The barley serine/threonine kinase gene Rpg1 providing resistance to stem rust belongs to a gene family with five other members encoding kinase domains.
    Article Snippet: The barley (Hordeum vulgare L.) stem rust (Puccinia graminis f. sp. tritici) resistance gene Rpg1 encodes a serine/threonine protein kinase with two tandem kinase domains.. The Rpg1 gene family was identiWed from the cv.. Morex and consists of Wve additional members with divergent homology to Rpg1.

    Article Title: Expression of a Novel Leishmania Gene Encoding a Histone H1-Like Protein in Leishmania major Modulates Parasite Infectivity In Vitro
    Article Snippet: An L. major cDNA library was constructed in the lambda Uni-ZAP XR vector as described in the technical manual provided by Stratagene Inc. (La Jolla, Calif.).

    Article Title: Genes expressed in zoospores of Phytophthora nicotianae.
    Article Snippet: The genus Phytophthora includes many highly destructive plant pathogens.. In many Phytophthora species, pathogen dispersal and initiation of plant infection are achieved by motile, biflagellate zoospores that are chemotactically attracted to suitable infection sites.. In order to study gene expression in zoospores, we have constructed a cDNA library using mRNA from zoospores of Phytophthora nicotianae.

    Plasmid Preparation:

    Article Title: Defining the phosphite-regulated transcriptome of the plant pathogen Phytophthora cinnamomi.
    Article Snippet: Phosphite, an analog of phosphate is used to control oomycete diseases on a wide range of horticultural crops and in native ecosystems.. In this study, we investigated morphological and transcriptional changes induced in Phytophthora cinnamomi by phosphite.. Cytological observations revealed that phosphite caused hyphal distortions and lysis of cell walls and had an adverse effect on hyphal growth.

    Article Title: Characterization of a novel Taenia solium oncosphere antigen
    Article Snippet: The cDNA was cloned into the lambda Uni-ZAP XR vector followed by packaging of the lambda DNA using the Gigapack® III Gold Cloning Kit (Stratagene) according to manufacturer’s instructions.

    Article Title: Hamster sperm protein, p26h: a member of the short-chain dehydrogenase/reductase superfamily.
    Article Snippet: In brief, testicular poly(A)1 RNA was reverse transcribed and ligated into the lambda Uni-Zap XR vector (Stratagene, La Jolla, CA).

    Article Title: A genomic survey of the fish parasite Spironucleus salmonicida indicates genomic plasticity among diplomonads and significant lateral gene transfer in eukaryote genome evolution
    Article Snippet: Messenger RNA from approximately 10 8 cells of S. salmonicida was isolated using the Dynabead mRNA system (Dynal), and cDNA was synthesized and cloned into the lambda Uni-Zap XR vector (Stratagene), according to the manufacturer's instructions.

    Article Title: Characterisation of tumour-associated antigens in colon cancer.
    Article Snippet: In order to search for clinically relevant cancerassociated genes and to define further the spectrum of immunogenic proteins, we applied SEREX (serological identification of antigens by recombinant expression cloning) to analyse genes expressed in colon adenocarcinoma.. Eight different serum-reactive cDNA clones were isolated by immunoscreening froma colon cancer-derived cDNA expression library. mRNA expression studies showed that 2 of them, RHAMM and AD034, have a differential tissue distribution, and that 3 genes, NAP1L1, RHAMM and AD034, are overexpressed in tumours in comparison with the adjacent non-cancerous tissues.. 5¢ RLM-RACE analysis of AD034, a sequence with a tyrosine kinase motif, revealed a frameshifting insertion of 32 bp, most likely generated by use of cryptic splice site in tumour-derived cDNA.

    Article Title: The barley serine/threonine kinase gene Rpg1 providing resistance to stem rust belongs to a gene family with five other members encoding kinase domains.
    Article Snippet: The barley (Hordeum vulgare L.) stem rust (Puccinia graminis f. sp. tritici) resistance gene Rpg1 encodes a serine/threonine protein kinase with two tandem kinase domains.. The Rpg1 gene family was identiWed from the cv.. Morex and consists of Wve additional members with divergent homology to Rpg1.

    Article Title: Expression of a Novel Leishmania Gene Encoding a Histone H1-Like Protein in Leishmania major Modulates Parasite Infectivity In Vitro
    Article Snippet: An L. major cDNA library was constructed in the lambda Uni-ZAP XR vector as described in the technical manual provided by Stratagene Inc. (La Jolla, Calif.).

    Article Title: Genes expressed in zoospores of Phytophthora nicotianae.
    Article Snippet: The genus Phytophthora includes many highly destructive plant pathogens.. In many Phytophthora species, pathogen dispersal and initiation of plant infection are achieved by motile, biflagellate zoospores that are chemotactically attracted to suitable infection sites.. In order to study gene expression in zoospores, we have constructed a cDNA library using mRNA from zoospores of Phytophthora nicotianae.

    Chromatography:

    Article Title: Defining the phosphite-regulated transcriptome of the plant pathogen Phytophthora cinnamomi.
    Article Snippet: Phosphite, an analog of phosphate is used to control oomycete diseases on a wide range of horticultural crops and in native ecosystems.. In this study, we investigated morphological and transcriptional changes induced in Phytophthora cinnamomi by phosphite.. Cytological observations revealed that phosphite caused hyphal distortions and lysis of cell walls and had an adverse effect on hyphal growth.

    Article Title: Characterization of a novel Taenia solium oncosphere antigen
    Article Snippet: The cDNA was cloned into the lambda Uni-ZAP XR vector followed by packaging of the lambda DNA using the Gigapack® III Gold Cloning Kit (Stratagene) according to manufacturer’s instructions.

    Article Title: Hamster sperm protein, p26h: a member of the short-chain dehydrogenase/reductase superfamily.
    Article Snippet: In brief, testicular poly(A)1 RNA was reverse transcribed and ligated into the lambda Uni-Zap XR vector (Stratagene, La Jolla, CA).

    Article Title: A genomic survey of the fish parasite Spironucleus salmonicida indicates genomic plasticity among diplomonads and significant lateral gene transfer in eukaryote genome evolution
    Article Snippet: Messenger RNA from approximately 10 8 cells of S. salmonicida was isolated using the Dynabead mRNA system (Dynal), and cDNA was synthesized and cloned into the lambda Uni-Zap XR vector (Stratagene), according to the manufacturer's instructions.

    Article Title: Characterisation of tumour-associated antigens in colon cancer.
    Article Snippet: In order to search for clinically relevant cancerassociated genes and to define further the spectrum of immunogenic proteins, we applied SEREX (serological identification of antigens by recombinant expression cloning) to analyse genes expressed in colon adenocarcinoma.. Eight different serum-reactive cDNA clones were isolated by immunoscreening froma colon cancer-derived cDNA expression library. mRNA expression studies showed that 2 of them, RHAMM and AD034, have a differential tissue distribution, and that 3 genes, NAP1L1, RHAMM and AD034, are overexpressed in tumours in comparison with the adjacent non-cancerous tissues.. 5¢ RLM-RACE analysis of AD034, a sequence with a tyrosine kinase motif, revealed a frameshifting insertion of 32 bp, most likely generated by use of cryptic splice site in tumour-derived cDNA.

    Article Title: The barley serine/threonine kinase gene Rpg1 providing resistance to stem rust belongs to a gene family with five other members encoding kinase domains.
    Article Snippet: The barley (Hordeum vulgare L.) stem rust (Puccinia graminis f. sp. tritici) resistance gene Rpg1 encodes a serine/threonine protein kinase with two tandem kinase domains.. The Rpg1 gene family was identiWed from the cv.. Morex and consists of Wve additional members with divergent homology to Rpg1.

    Article Title: Expression of a Novel Leishmania Gene Encoding a Histone H1-Like Protein in Leishmania major Modulates Parasite Infectivity In Vitro
    Article Snippet: An L. major cDNA library was constructed in the lambda Uni-ZAP XR vector as described in the technical manual provided by Stratagene Inc. (La Jolla, Calif.).

    Article Title: Genes expressed in zoospores of Phytophthora nicotianae.
    Article Snippet: The genus Phytophthora includes many highly destructive plant pathogens.. In many Phytophthora species, pathogen dispersal and initiation of plant infection are achieved by motile, biflagellate zoospores that are chemotactically attracted to suitable infection sites.. In order to study gene expression in zoospores, we have constructed a cDNA library using mRNA from zoospores of Phytophthora nicotianae.

    Lambda DNA Preparation:

    Article Title: Defining the phosphite-regulated transcriptome of the plant pathogen Phytophthora cinnamomi.
    Article Snippet: Phosphite, an analog of phosphate is used to control oomycete diseases on a wide range of horticultural crops and in native ecosystems.. In this study, we investigated morphological and transcriptional changes induced in Phytophthora cinnamomi by phosphite.. Cytological observations revealed that phosphite caused hyphal distortions and lysis of cell walls and had an adverse effect on hyphal growth.

    Article Title: Characterization of a novel Taenia solium oncosphere antigen
    Article Snippet: The cDNA was cloned into the lambda Uni-ZAP XR vector followed by packaging of the lambda DNA using the Gigapack® III Gold Cloning Kit (Stratagene) according to manufacturer’s instructions.

    Article Title: Hamster sperm protein, p26h: a member of the short-chain dehydrogenase/reductase superfamily.
    Article Snippet: In brief, testicular poly(A)1 RNA was reverse transcribed and ligated into the lambda Uni-Zap XR vector (Stratagene, La Jolla, CA).

    Article Title: A genomic survey of the fish parasite Spironucleus salmonicida indicates genomic plasticity among diplomonads and significant lateral gene transfer in eukaryote genome evolution
    Article Snippet: Messenger RNA from approximately 10 8 cells of S. salmonicida was isolated using the Dynabead mRNA system (Dynal), and cDNA was synthesized and cloned into the lambda Uni-Zap XR vector (Stratagene), according to the manufacturer's instructions.

    Article Title: Characterisation of tumour-associated antigens in colon cancer.
    Article Snippet: In order to search for clinically relevant cancerassociated genes and to define further the spectrum of immunogenic proteins, we applied SEREX (serological identification of antigens by recombinant expression cloning) to analyse genes expressed in colon adenocarcinoma.. Eight different serum-reactive cDNA clones were isolated by immunoscreening froma colon cancer-derived cDNA expression library. mRNA expression studies showed that 2 of them, RHAMM and AD034, have a differential tissue distribution, and that 3 genes, NAP1L1, RHAMM and AD034, are overexpressed in tumours in comparison with the adjacent non-cancerous tissues.. 5¢ RLM-RACE analysis of AD034, a sequence with a tyrosine kinase motif, revealed a frameshifting insertion of 32 bp, most likely generated by use of cryptic splice site in tumour-derived cDNA.

    Article Title: The barley serine/threonine kinase gene Rpg1 providing resistance to stem rust belongs to a gene family with five other members encoding kinase domains.
    Article Snippet: The barley (Hordeum vulgare L.) stem rust (Puccinia graminis f. sp. tritici) resistance gene Rpg1 encodes a serine/threonine protein kinase with two tandem kinase domains.. The Rpg1 gene family was identiWed from the cv.. Morex and consists of Wve additional members with divergent homology to Rpg1.

    Article Title: Expression of a Novel Leishmania Gene Encoding a Histone H1-Like Protein in Leishmania major Modulates Parasite Infectivity In Vitro
    Article Snippet: An L. major cDNA library was constructed in the lambda Uni-ZAP XR vector as described in the technical manual provided by Stratagene Inc. (La Jolla, Calif.).

    Article Title: Genes expressed in zoospores of Phytophthora nicotianae.
    Article Snippet: The genus Phytophthora includes many highly destructive plant pathogens.. In many Phytophthora species, pathogen dispersal and initiation of plant infection are achieved by motile, biflagellate zoospores that are chemotactically attracted to suitable infection sites.. In order to study gene expression in zoospores, we have constructed a cDNA library using mRNA from zoospores of Phytophthora nicotianae.

    Hybridization:

    Article Title: Defining the phosphite-regulated transcriptome of the plant pathogen Phytophthora cinnamomi.
    Article Snippet: Phosphite, an analog of phosphate is used to control oomycete diseases on a wide range of horticultural crops and in native ecosystems.. In this study, we investigated morphological and transcriptional changes induced in Phytophthora cinnamomi by phosphite.. Cytological observations revealed that phosphite caused hyphal distortions and lysis of cell walls and had an adverse effect on hyphal growth.

    Article Title: Characterization of a novel Taenia solium oncosphere antigen
    Article Snippet: The cDNA was cloned into the lambda Uni-ZAP XR vector followed by packaging of the lambda DNA using the Gigapack® III Gold Cloning Kit (Stratagene) according to manufacturer’s instructions.

    Article Title: Hamster sperm protein, p26h: a member of the short-chain dehydrogenase/reductase superfamily.
    Article Snippet: In brief, testicular poly(A)1 RNA was reverse transcribed and ligated into the lambda Uni-Zap XR vector (Stratagene, La Jolla, CA).

    Article Title: A genomic survey of the fish parasite Spironucleus salmonicida indicates genomic plasticity among diplomonads and significant lateral gene transfer in eukaryote genome evolution
    Article Snippet: Messenger RNA from approximately 10 8 cells of S. salmonicida was isolated using the Dynabead mRNA system (Dynal), and cDNA was synthesized and cloned into the lambda Uni-Zap XR vector (Stratagene), according to the manufacturer's instructions.

    Article Title: Characterisation of tumour-associated antigens in colon cancer.
    Article Snippet: In order to search for clinically relevant cancerassociated genes and to define further the spectrum of immunogenic proteins, we applied SEREX (serological identification of antigens by recombinant expression cloning) to analyse genes expressed in colon adenocarcinoma.. Eight different serum-reactive cDNA clones were isolated by immunoscreening froma colon cancer-derived cDNA expression library. mRNA expression studies showed that 2 of them, RHAMM and AD034, have a differential tissue distribution, and that 3 genes, NAP1L1, RHAMM and AD034, are overexpressed in tumours in comparison with the adjacent non-cancerous tissues.. 5¢ RLM-RACE analysis of AD034, a sequence with a tyrosine kinase motif, revealed a frameshifting insertion of 32 bp, most likely generated by use of cryptic splice site in tumour-derived cDNA.

    Article Title: The barley serine/threonine kinase gene Rpg1 providing resistance to stem rust belongs to a gene family with five other members encoding kinase domains.
    Article Snippet: The barley (Hordeum vulgare L.) stem rust (Puccinia graminis f. sp. tritici) resistance gene Rpg1 encodes a serine/threonine protein kinase with two tandem kinase domains.. The Rpg1 gene family was identiWed from the cv.. Morex and consists of Wve additional members with divergent homology to Rpg1.

    Article Title: Expression of a Novel Leishmania Gene Encoding a Histone H1-Like Protein in Leishmania major Modulates Parasite Infectivity In Vitro
    Article Snippet: An L. major cDNA library was constructed in the lambda Uni-ZAP XR vector as described in the technical manual provided by Stratagene Inc. (La Jolla, Calif.).

    Article Title: Genes expressed in zoospores of Phytophthora nicotianae.
    Article Snippet: The genus Phytophthora includes many highly destructive plant pathogens.. In many Phytophthora species, pathogen dispersal and initiation of plant infection are achieved by motile, biflagellate zoospores that are chemotactically attracted to suitable infection sites.. In order to study gene expression in zoospores, we have constructed a cDNA library using mRNA from zoospores of Phytophthora nicotianae.

    cDNA Library Assay:

    Article Title: Defining the phosphite-regulated transcriptome of the plant pathogen Phytophthora cinnamomi.
    Article Snippet: Phosphite, an analog of phosphate is used to control oomycete diseases on a wide range of horticultural crops and in native ecosystems.. In this study, we investigated morphological and transcriptional changes induced in Phytophthora cinnamomi by phosphite.. Cytological observations revealed that phosphite caused hyphal distortions and lysis of cell walls and had an adverse effect on hyphal growth.

    Article Title: Characterization of a novel Taenia solium oncosphere antigen
    Article Snippet: The cDNA was cloned into the lambda Uni-ZAP XR vector followed by packaging of the lambda DNA using the Gigapack® III Gold Cloning Kit (Stratagene) according to manufacturer’s instructions.

    Article Title: Hamster sperm protein, p26h: a member of the short-chain dehydrogenase/reductase superfamily.
    Article Snippet: In brief, testicular poly(A)1 RNA was reverse transcribed and ligated into the lambda Uni-Zap XR vector (Stratagene, La Jolla, CA).

    Article Title: A genomic survey of the fish parasite Spironucleus salmonicida indicates genomic plasticity among diplomonads and significant lateral gene transfer in eukaryote genome evolution
    Article Snippet: Messenger RNA from approximately 10 8 cells of S. salmonicida was isolated using the Dynabead mRNA system (Dynal), and cDNA was synthesized and cloned into the lambda Uni-Zap XR vector (Stratagene), according to the manufacturer's instructions.

    Article Title: Characterisation of tumour-associated antigens in colon cancer.
    Article Snippet: In order to search for clinically relevant cancerassociated genes and to define further the spectrum of immunogenic proteins, we applied SEREX (serological identification of antigens by recombinant expression cloning) to analyse genes expressed in colon adenocarcinoma.. Eight different serum-reactive cDNA clones were isolated by immunoscreening froma colon cancer-derived cDNA expression library. mRNA expression studies showed that 2 of them, RHAMM and AD034, have a differential tissue distribution, and that 3 genes, NAP1L1, RHAMM and AD034, are overexpressed in tumours in comparison with the adjacent non-cancerous tissues.. 5¢ RLM-RACE analysis of AD034, a sequence with a tyrosine kinase motif, revealed a frameshifting insertion of 32 bp, most likely generated by use of cryptic splice site in tumour-derived cDNA.

    Article Title: The barley serine/threonine kinase gene Rpg1 providing resistance to stem rust belongs to a gene family with five other members encoding kinase domains.
    Article Snippet: The barley (Hordeum vulgare L.) stem rust (Puccinia graminis f. sp. tritici) resistance gene Rpg1 encodes a serine/threonine protein kinase with two tandem kinase domains.. The Rpg1 gene family was identiWed from the cv.. Morex and consists of Wve additional members with divergent homology to Rpg1.

    Article Title: Expression of a Novel Leishmania Gene Encoding a Histone H1-Like Protein in Leishmania major Modulates Parasite Infectivity In Vitro
    Article Snippet: An L. major cDNA library was constructed in the lambda Uni-ZAP XR vector as described in the technical manual provided by Stratagene Inc. (La Jolla, Calif.).

    Article Title: Genes expressed in zoospores of Phytophthora nicotianae.
    Article Snippet: The genus Phytophthora includes many highly destructive plant pathogens.. In many Phytophthora species, pathogen dispersal and initiation of plant infection are achieved by motile, biflagellate zoospores that are chemotactically attracted to suitable infection sites.. In order to study gene expression in zoospores, we have constructed a cDNA library using mRNA from zoospores of Phytophthora nicotianae.

    Construct:

    Article Title: Defining the phosphite-regulated transcriptome of the plant pathogen Phytophthora cinnamomi.
    Article Snippet: Phosphite, an analog of phosphate is used to control oomycete diseases on a wide range of horticultural crops and in native ecosystems.. In this study, we investigated morphological and transcriptional changes induced in Phytophthora cinnamomi by phosphite.. Cytological observations revealed that phosphite caused hyphal distortions and lysis of cell walls and had an adverse effect on hyphal growth.

    Article Title: Characterization of a novel Taenia solium oncosphere antigen
    Article Snippet: The cDNA was cloned into the lambda Uni-ZAP XR vector followed by packaging of the lambda DNA using the Gigapack® III Gold Cloning Kit (Stratagene) according to manufacturer’s instructions.

    Article Title: Hamster sperm protein, p26h: a member of the short-chain dehydrogenase/reductase superfamily.
    Article Snippet: In brief, testicular poly(A)1 RNA was reverse transcribed and ligated into the lambda Uni-Zap XR vector (Stratagene, La Jolla, CA).

    Article Title: A genomic survey of the fish parasite Spironucleus salmonicida indicates genomic plasticity among diplomonads and significant lateral gene transfer in eukaryote genome evolution
    Article Snippet: Messenger RNA from approximately 10 8 cells of S. salmonicida was isolated using the Dynabead mRNA system (Dynal), and cDNA was synthesized and cloned into the lambda Uni-Zap XR vector (Stratagene), according to the manufacturer's instructions.

    Article Title: Characterisation of tumour-associated antigens in colon cancer.
    Article Snippet: In order to search for clinically relevant cancerassociated genes and to define further the spectrum of immunogenic proteins, we applied SEREX (serological identification of antigens by recombinant expression cloning) to analyse genes expressed in colon adenocarcinoma.. Eight different serum-reactive cDNA clones were isolated by immunoscreening froma colon cancer-derived cDNA expression library. mRNA expression studies showed that 2 of them, RHAMM and AD034, have a differential tissue distribution, and that 3 genes, NAP1L1, RHAMM and AD034, are overexpressed in tumours in comparison with the adjacent non-cancerous tissues.. 5¢ RLM-RACE analysis of AD034, a sequence with a tyrosine kinase motif, revealed a frameshifting insertion of 32 bp, most likely generated by use of cryptic splice site in tumour-derived cDNA.

    Article Title: The barley serine/threonine kinase gene Rpg1 providing resistance to stem rust belongs to a gene family with five other members encoding kinase domains.
    Article Snippet: The barley (Hordeum vulgare L.) stem rust (Puccinia graminis f. sp. tritici) resistance gene Rpg1 encodes a serine/threonine protein kinase with two tandem kinase domains.. The Rpg1 gene family was identiWed from the cv.. Morex and consists of Wve additional members with divergent homology to Rpg1.

    Article Title: Expression of a Novel Leishmania Gene Encoding a Histone H1-Like Protein in Leishmania major Modulates Parasite Infectivity In Vitro
    Article Snippet: An L. major cDNA library was constructed in the lambda Uni-ZAP XR vector as described in the technical manual provided by Stratagene Inc. (La Jolla, Calif.).

    Article Title: Genes expressed in zoospores of Phytophthora nicotianae.
    Article Snippet: The genus Phytophthora includes many highly destructive plant pathogens.. In many Phytophthora species, pathogen dispersal and initiation of plant infection are achieved by motile, biflagellate zoospores that are chemotactically attracted to suitable infection sites.. In order to study gene expression in zoospores, we have constructed a cDNA library using mRNA from zoospores of Phytophthora nicotianae.

    Purification:

    Article Title: Defining the phosphite-regulated transcriptome of the plant pathogen Phytophthora cinnamomi.
    Article Snippet: Phosphite, an analog of phosphate is used to control oomycete diseases on a wide range of horticultural crops and in native ecosystems.. In this study, we investigated morphological and transcriptional changes induced in Phytophthora cinnamomi by phosphite.. Cytological observations revealed that phosphite caused hyphal distortions and lysis of cell walls and had an adverse effect on hyphal growth.

    Article Title: Characterization of a novel Taenia solium oncosphere antigen
    Article Snippet: The cDNA was cloned into the lambda Uni-ZAP XR vector followed by packaging of the lambda DNA using the Gigapack® III Gold Cloning Kit (Stratagene) according to manufacturer’s instructions.

    Article Title: Hamster sperm protein, p26h: a member of the short-chain dehydrogenase/reductase superfamily.
    Article Snippet: In brief, testicular poly(A)1 RNA was reverse transcribed and ligated into the lambda Uni-Zap XR vector (Stratagene, La Jolla, CA).

    Article Title: A genomic survey of the fish parasite Spironucleus salmonicida indicates genomic plasticity among diplomonads and significant lateral gene transfer in eukaryote genome evolution
    Article Snippet: Messenger RNA from approximately 10 8 cells of S. salmonicida was isolated using the Dynabead mRNA system (Dynal), and cDNA was synthesized and cloned into the lambda Uni-Zap XR vector (Stratagene), according to the manufacturer's instructions.

    Article Title: Characterisation of tumour-associated antigens in colon cancer.
    Article Snippet: In order to search for clinically relevant cancerassociated genes and to define further the spectrum of immunogenic proteins, we applied SEREX (serological identification of antigens by recombinant expression cloning) to analyse genes expressed in colon adenocarcinoma.. Eight different serum-reactive cDNA clones were isolated by immunoscreening froma colon cancer-derived cDNA expression library. mRNA expression studies showed that 2 of them, RHAMM and AD034, have a differential tissue distribution, and that 3 genes, NAP1L1, RHAMM and AD034, are overexpressed in tumours in comparison with the adjacent non-cancerous tissues.. 5¢ RLM-RACE analysis of AD034, a sequence with a tyrosine kinase motif, revealed a frameshifting insertion of 32 bp, most likely generated by use of cryptic splice site in tumour-derived cDNA.

    Article Title: The barley serine/threonine kinase gene Rpg1 providing resistance to stem rust belongs to a gene family with five other members encoding kinase domains.
    Article Snippet: The barley (Hordeum vulgare L.) stem rust (Puccinia graminis f. sp. tritici) resistance gene Rpg1 encodes a serine/threonine protein kinase with two tandem kinase domains.. The Rpg1 gene family was identiWed from the cv.. Morex and consists of Wve additional members with divergent homology to Rpg1.

    Article Title: Expression of a Novel Leishmania Gene Encoding a Histone H1-Like Protein in Leishmania major Modulates Parasite Infectivity In Vitro
    Article Snippet: An L. major cDNA library was constructed in the lambda Uni-ZAP XR vector as described in the technical manual provided by Stratagene Inc. (La Jolla, Calif.).

    Article Title: Genes expressed in zoospores of Phytophthora nicotianae.
    Article Snippet: The genus Phytophthora includes many highly destructive plant pathogens.. In many Phytophthora species, pathogen dispersal and initiation of plant infection are achieved by motile, biflagellate zoospores that are chemotactically attracted to suitable infection sites.. In order to study gene expression in zoospores, we have constructed a cDNA library using mRNA from zoospores of Phytophthora nicotianae.



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